Jonathan Magbie Remembrance: Endocannabinoid Pregnancy Science at 20 Years

9/24/2015

Jonathan Magbie Remembrance

Jonathan Magbie died eleven years ago today, 24 September 2004; in remembrance, this PubMed update spans 20 years of endocannabinoid pregnancy science, with an emphasis on preimplantation and implantation of a blastocyst.

Like all of us, Magbie was born (1977 for him) via a mother's cannabinoid system.

1995
The preimplantation mouse embryo is a target for cannabinoid ligand-receptor signaling.
Paria BC, Das SK, Dey SK.
Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9460-4.
PMID: 7568154 Free PMC Article
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1998
Effects of cannabinoids on preimplantation mouse embryo development and implantation are mediated by brain-type cannabinoid receptors.
Paria BC, Ma W, Andrenyak DM, Schmid PC, Schmid HH, Moody DE, Deng H, Makriyannis A, Dey SK.
Biol Reprod. 1998 Jun;58(6):1490-5.
PMID: 9623610 Free Article
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2000
Ligand-receptor signaling with endocannabinoids in preimplantation embryo development and implantation.
Paria BC, Dey SK.
Chem Phys Lipids. 2000 Nov;108(1-2):211-20. Review.
PMID: 11106792
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2002
Endocannabinoid signaling in synchronizing embryo development and uterine receptivity for implantation.
Paria BC, Wang H, Dey SK.
Chem Phys Lipids. 2002 Dec 31;121(1-2):201-10. Review.
PMID: 12505701
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2006
Endocannabinoid signaling directs periimplantation events.
Wang H, Xie H, Dey SK.
AAPS J. 2006;8(2):E425-32.
PMID: 16808046 Free PMC Article
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2008
Aspects of endocannabinoid signaling in periimplantation biology.
Sun X, Dey SK.
Mol Cell Endocrinol. 2008 Apr 16;286(1-2 Suppl 1):S3-11. doi: 10.1016/j.mce.2008.01.002. Epub 2008 Jan 18. Review.
PMID: 18294762 Free PMC Article
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2009
Endocannabinoids, sperm biology and human fertility.
Lewis SE, Maccarrone M.
Pharmacol Res. 2009 Aug;60(2):126-31. doi: 10.1016/j.phrs.2009.02.009. Epub 2009 Mar 4. Review.
PMID: 19559363
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2010
Endocannabinoids and pregnancy.
Taylor AH, Amoako AA, Bambang K, Karasu T, Gebeh A, Lam PM, Marzcylo TH, Konje JC.
Clin Chim Acta. 2010 Jul 4;411(13-14):921-30. doi: 10.1016/j.cca.2010.03.012. Epub 2010 Mar 17. Review.
PMID: 20302856
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2011
The manifold actions of endocannabinoids on female and male reproductive events.
Bari M, Battista N, Pirazzi V, Maccarrone M.
Front Biosci (Landmark Ed). 2011 Jan 1;16:498-516. Review.
PMID: 21196184
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2012
Silencing or amplification of endocannabinoid signaling in blastocysts via CB1 compromises trophoblast cell migration.
Xie H, Sun X, Piao Y, Jegga AG, Handwerger S, Ko MS, Dey SK.
J Biol Chem. 2012 Sep 14;287(38):32288-97. doi: 10.1074/jbc.M112.381145. Epub 2012 Jul 24.
PMID: 22833670 Free PMC Article
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2013
The endocannabinoid pathway and the female reproductive organs.
Di Blasio AM, Vignali M, Gentilini D.
J Mol Endocrinol. 2013 Jan 11;50(1):R1-9. doi: 10.1530/JME-12-0182. Print 2013 Feb. Review.
PMID: 23178290 Free Article
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2014
Endocannabinoid signaling in mammalian ovary.
Cecconi S, Rossi G, Castellucci A, D'Andrea G, Maccarrone M.
Eur J Obstet Gynecol Reprod Biol. 2014 Jul;178:6-11. doi: 10.1016/j.ejogrb.2014.04.011. Epub 2014 Apr 18. Review.
PMID: 24948047
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2015
Endocannabinoid signaling in female reproductive events: a potential therapeutic target?
Maccarrone M.
Expert Opin Ther Targets. 2015 Jun 30:1-5. [Epub ahead of print]
PMID: 26126134
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Peace.

*Next Up: Saturday 10 October and the first round of Whiskey221, a continuation of last year’s series Whiskey220 (it’s about George Washington, our Republic and whiskey rebels).

Posted by Bryan W. Brickner

4 Comments

Michael Nielsen link

10/6/2015 09:00:51 am

Namaste Brian William Brickner

Circulating Endocannabinoid Concentrations and Sexual Arousal in Women.

Carolin Klein, PhD,* Matthew N. Hill, PhD,† Sabrina C.H. Chang, BA,* Cecilia J. Hillard, PhD,‡ and Boris B. Gorzalka, PhD*

Introduction
Several lines of evidence point to the potential role of the endocannabinoid system in female sexual functioning. These include results from studies describing the subjective effects of exogenous cannabinoids on sexual functioning in humans and the observable effects of exogenous cannabinoids on sexual functioning in other species, as well as results from studies investigating the location of cannabinoid receptors in the brain and periphery, and the effects of cannabinoid receptor activation on neurotransmitters implicated in sexual functioning. While these lines of research suggest a role for the endocannabinoid system in female sexual functioning, no studies investigating the relationship between concentrations of endogenous cannabinoids (i.e., arachidonoylethanolamide [AEA] and 2-arachidonoylglycerol [2-AG]) and sexual functioning have been conducted in any species.

Aim
To measure circulating endocannabinoid concentrations in relation to subjective and physiological indices of sexual arousal in women (n = 21).

...

Results
Results revealed a significant relationship between endocannabinoid concentrations and female sexual arousal, whereby increases in both physiological and subjective indices of sexual arousal were significantly associated with decreases in AEA, and increases in subjective indices of sexual arousal were significantly associated with decreases in 2-AG.

Conclusions
These findings support the hypothesis that the endocannabinoid system is involved in female sexual functioning, with implications for furthering understanding of the biological mechanisms underlying female sexual functioning.

- J Sex Med. 2012 Jun; 9(6): 10.1111/j.1743-6109.2012.02708.x

* http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856894/

With best wishes and thank you

Michael Nielsen link

10/6/2015 09:24:20 am

Namaste Bryan William Brickner

Endocannabinoid signaling at the periphery: 50 years after THC.

Maccarrone M1, Bab I2, Bíró T3, Cabral GA4, Dey SK5, Di Marzo V6, Konje JC7, Kunos G8, Mechoulam R9, Pacher P8, Sharkey KA10, Zimmer A11.

Abstract

In 1964, the psychoactive ingredient of Cannabis sativa, Δ(9)-tetrahydrocannabinol (THC), was isolated. Nearly 30 years later the endogenous counterparts of THC, collectively termed endocannabinoids (eCBs), were discovered: N-arachidonoylethanolamine (anandamide) (AEA) in 1992 and 2-arachidonoylglycerol (2-AG) in 1995. ...

eCBs control basic biological processes including cell choice between survival and death and progenitor/stem cell proliferation and differentiation. Unsurprisingly, in the past two decades eCBs have been recognized as key mediators of several aspects of human pathophysiology and thus have emerged to be among the most widespread and versatile signaling molecules ever discovered. ...

Trends Pharmacol Sci. 2015 May;36(5):277-96. doi: 10.1016

* https://www.ncbi.nlm.nih.gov/pubmed/25796370

Best wishes and thank you

Michael Nielsen link

10/9/2015 02:49:19 am

Namaste Bryan William Brickner.

A welcome to life and supported transition...

Endocannabinoid Levels in Newborns in Relation to the Mode of Delivery -

Jokisch V, Kroll R, Lutz B, Bindila L, Schaffelder R, Schaible T.

Objective: This study aims to examine the endocannabinoid levels in newborns in relation to the mode of delivery.

Study Design: In this study, the concentrations of the endocannabinoids anandamide (AEA), 2-arachidonylglycerol (2-AG), palmitoylethanolamine (PEA), and the metabolite arachidonic acid (AA) in umbilical cord vein blood of 49 newborns was determined by quantitative mass spectrometry using liquid chromatography multiple reaction monitoring.

The newborns were divided by their mode of delivery. Only healthy newborns born after 340/7 gestational weeks without birth complications were included.

Results: The concentration of AEA, PEA, and AA was significantly higher in vaginal delivered newborns (n=25) in comparison to newborns born by cesarean delivery (n=24). 2-AG exhibited no significant differences between the groups.

Conclusion: The exposure of the newborn to high endocannabinoid concentrations is a physiological process during vaginal delivery. The endocannabinoids AEA, PEA, and their metabolite AA seem to be part of an endocrine system during labor and birth supporting the fetal transition.

Am J Perinatol. 2015 Oct;32(12):1145-1150.

* https://www.ncbi.nlm.nih.gov/pubmed/25915139

With best wishes and thank You

Michael Nielsen link

10/9/2015 04:32:27 am

Namaste Bryan William Brickner.

In relation...

Anxiety, Stress, and Fear Response in Mice with Reduced Endocannabinoid Levels

Jenniches I, Ternes S, Albayram O, Otte DM, Bach K, Bindila L, Michel K, Lutz B, Bilkei-Gorzo A, Zimmer A.

BACKGROUND: Disruption of the endocannabinoid system through pharmacological or genetic invalidation of cannabinoid CB1 receptors has been linked to depression in humans and depression-like behaviors in mice. The two main endogenous cannabinoids, anandamide and 2-arachidonoyl glycerol (2-AG), are produced on demand from phospholipids. The pathways and enzymes involved in endocannabinoid biosynthesis thus play a major role in regulating the activity of this system. This study investigates the role of the main 2-AG producing enzyme diacylglycerol lipase α (DAGL-α).

METHODS: We generated and used knockout mice lacking DAGL-α (Dagla-/-) to assess the behavioral consequences of reduced endocannabinoid levels in the brain. We performed different behavior tests to determine anxiety- and depression-related behavioral changes in Dagla-/- mice.

We also analyzed expression of genes related to the endocannabinoid system via real-time polymerase chain reaction and used the mitotic marker 5-bromo-2'-deoxyuridine to analyze adult neurogenesis.

RESULTS: Dagla-/- animals show an 80% reduction of brain 2-AG levels but also a reduction in cortical and amygdalar anandamide. The behavioral changes induced by Dagla deletion include a reduced exploration of the central area of the open field, a maternal neglect behavior, a fear extinction deficit, increased behavioral despair, increased anxiety-related behaviors in the light/dark box, and reduced hippocampal neurogenesis. Some of these behavioral changes resemble those observed in animals lacking the CB1 receptor.

CONCLUSIONS: Our findings demonstrate that the deletion of Dagla adversely affects the emotional state of animals and results in enhanced anxiety, stress, and fear responses.

Biol Psychiatry. 2015 Apr 14. pii: S0006-3223(15)00314-5. doi: 10.1016

* http://www.ncbi.nlm.nih.gov/pubmed/25981172

With best wishes

Jonathan Magbie Remembrance: Endocannabinoid Pregnancy Science at 20 Years

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